POLD3

Protein-coding gene in the species Homo sapiens

POLD3

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
1U76, 3E0J, 2N1G

Identifiers

Aliases

POLD3, P66, P68, PPP1R128, polymerase (DNA) delta 3, accessory subunit, DNA polymerase delta 3, accessory subunit

External IDs

OMIM: 611415; MGI: 1915217; HomoloGene: 38202; GeneCards: POLD3; OMA:POLD3 - orthologs

Gene location (Human)

Chr.	Chromosome 11 (human)^[1]

Band	11q13.4	Start	74,493,851 bp^[1]
Band	11q13.4	End	74,669,117 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 7 (mouse)^[2]

Band	7\|7 E2	Start	99,731,318 bp^[2]
Band	7\|7 E2	End	99,770,772 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

secondary oocyte

ventricular zone

testicle

sperm

ganglionic eminence

rectum

gonad

monocyte

kidney tubule

bronchial epithelial cell

Top expressed in

secondary oocyte

zygote

tail of embryo

primary oocyte

genital tubercle

spermatid

morula

somite

primitive streak

seminiferous tubule

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	protein binding DNA-directed DNA polymerase activity
Cellular component	delta DNA polymerase complex nucleus nucleoplasm cytoplasm
Biological process	nucleotide-excision repair, DNA gap filling DNA synthesis involved in DNA repair DNA replication translesion synthesis transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision telomere maintenance DNA-dependent DNA replication nucleotide-excision repair, DNA incision, 5'-to lesion DNA repair cellular response to DNA damage stimulus telomere maintenance via semi-conservative replication DNA mismatch repair DNA strand elongation involved in DNA replication DNA synthesis involved in UV-damage excision repair
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


10714


67967

Ensembl


ENSG00000077514


ENSMUSG00000030726

UniProt


Q15054


Q9EQ28

RefSeq (mRNA)


NM_006591 NM_001363597


NM_133692 NM_001368380

RefSeq (protein)


NP_006582 NP_001350526


NP_598453 NP_001355309

Location (UCSC)

Chr 11: 74.49 – 74.67 Mb

Chr 7: 99.73 – 99.77 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

DNA polymerase delta subunit 3 is an enzyme that in humans is encoded by the POLD3 gene.^[5]^[6] It is a component of the DNA polymerase delta complex.

Interactions

POLD3 has been shown to interact with PCNA.^[7]^[8]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000077514 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000030726 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Hughes P, Tratner I, Ducoux M, Piard K, Baldacci G (Jun 1999). "Isolation and identification of the third subunit of mammalian DNA polymerase delta by PCNA-affinity chromatography of mouse FM3A cell extracts". Nucleic Acids Res. 27 (10): 2108–14. doi:10.1093/nar/27.10.2108. PMC 148430. PMID 10219083.
^ "Entrez Gene: POLD3 polymerase (DNA-directed), delta 3, accessory subunit".
^ Ducoux M, Urbach S, Baldacci G, Hübscher U, Koundrioukoff S, Christensen J, Hughes P (Dec 2001). "Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta". J. Biol. Chem. 276 (52): 49258–66. doi:10.1074/jbc.M106990200. PMID 11595739.
^ Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.

Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S (1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1". DNA Res. 1 (1): 27–35. doi:10.1093/dnares/1.1.27. PMID 7584026.
Zeng XR, Jiang Y, Zhang SJ, Hao H, Lee MY (1994). "DNA polymerase delta is involved in the cellular response to UV damage in human cells". J. Biol. Chem. 269 (19): 13748–51. doi:10.1016/S0021-9258(17)36709-1. PMID 7910606.
Longley MJ, Pierce AJ, Modrich P (1997). "DNA polymerase delta is required for human mismatch repair in vitro". J. Biol. Chem. 272 (16): 10917–21. doi:10.1074/jbc.272.16.10917. PMID 9099749.
Mo J, Liu L, Leon A, Mazloum N, Lee MY (2000). "Evidence that DNA polymerase delta isolated by immunoaffinity chromatography exhibits high-molecular weight characteristics and is associated with the KIAA0039 protein and RPA". Biochemistry. 39 (24): 7245–54. doi:10.1021/bi0000871. PMID 10852724.
Ducoux M, Urbach S, Baldacci G, Hübscher U, Koundrioukoff S, Christensen J, Hughes P (2002). "Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta". J. Biol. Chem. 276 (52): 49258–66. doi:10.1074/jbc.M106990200. PMID 11595739.
Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.
Pohler JR, Otterlei M, Warbrick E (2006). "An in vivo analysis of the localisation and interactions of human p66 DNA polymerase delta subunit". BMC Mol. Biol. 6: 17. doi:10.1186/1471-2199-6-17. PMC 1187890. PMID 16000169.
Takemura M, Yoshida S, Akiyama T, Kitagawa M, Yamada Y (2006). "Role of the second-largest subunit of DNA polymerase alpha in the interaction between the catalytic subunit and hyperphosphorylated retinoblastoma protein in late S phase". Biochim. Biophys. Acta. 1764 (9): 1447–53. doi:10.1016/j.bbapap.2006.06.015. PMID 16935576.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
Masuda Y, Suzuki M, Piao J, Gu Y, Tsurimoto T, Kamiya K (2007). "Dynamics of human replication factors in the elongation phase of DNA replication". Nucleic Acids Res. 35 (20): 6904–16. doi:10.1093/nar/gkm822. PMC 2175312. PMID 17932049.

DNA replication (comparing prokaryotic to eukaryotic)

Initiation

Prokaryotic (initiation)	Pre-replication complex dnaC Helicase dnaA dnaB T7 Primase dnaG
Eukaryotic (preparation in G1 phase)	Pre-replication complex Origin recognition complex ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 Cdc6 Cdt1 Minichromosome maintenance MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 Licensing factor Autonomously replicating sequence Single-strand binding protein SSBP2 SSBP3 SSBP4 RNase H RNASEH1 RNASEH2A Helicase: HFM1 Primase: PRIM1 PRIM2
Both	Origin of replication/Ori/Replicon Replication fork Lagging and leading strands Okazaki fragments Primer

Replication

Prokaryotic (elongation)	DNA polymerase III holoenzyme dnaC dnaE dnaH dnaN dnaQ dnaT dnaX holA holB holC holD holE Replisome DNA ligase DNA clamp Topoisomerase DNA gyrase Prokaryotic DNA polymerase: DNA polymerase I Klenow fragment
Eukaryotic (synthesis in S phase)	Replication factor C RFC1 Flap endonuclease FEN1 Topoisomerase Replication protein A RPA1 Eukaryotic DNA polymerase: alpha POLA1 POLA2 PRIM1 PRIM2 delta POLD1 POLD2 POLD3 POLD4 epsilon POLE POLE2 POLE3 POLE4 DNA clamp PCNA Control of chromosome duplication
Both	Movement: Processivity DNA ligase