VPS28

Protein-coding gene in the species Homo sapiens

VPS28

Identifiers

Aliases

VPS28, ESCRT-I subunit, VPS28 subunit of ESCRT-I

External IDs

OMIM: 611952; MGI: 1914164; HomoloGene: 69205; GeneCards: VPS28; OMA:VPS28 - orthologs

Gene location (Human)

Chr.	Chromosome 8 (human)^[1]

Band	8q24.3	Start	144,423,601 bp^[1]
Band	8q24.3	End	144,428,563 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 15 (mouse)^[2]

Band	15\|15 D3	Start	76,506,287 bp^[2]
Band	15\|15 D3	End	76,510,286 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

right uterine tube

left testis

apex of heart

right lobe of thyroid gland

right ovary

left ovary

duodenum

fundus

right testis

left lobe of thyroid gland

Top expressed in

ankle joint

facial motor nucleus

right lung lobe

central gray substance of midbrain

vestibular membrane of cochlear duct

anterior horn of spinal cord

transitional epithelium of urinary bladder

nucleus of stria terminalis

granulocyte

adrenal gland

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	protein-containing complex binding ubiquitin binding protein binding
Cellular component	cytoplasm cytosol endosome membrane late endosome membrane plasma membrane early endosome endosome membrane extracellular exosome ESCRT I complex host cell
Biological process	intracellular transport of virus positive regulation of ubiquitin-dependent endocytosis positive regulation of protein catabolic process viral budding via host ESCRT complex viral life cycle multivesicular body assembly endosomal transport negative regulation of protein ubiquitination protein transport ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway macroautophagy transport endosome transport via multivesicular body sorting pathway
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


51160


66914

Ensembl


ENSG00000160948 ENSG00000285339


ENSMUSG00000115987

UniProt


Q9UK41 Q548N1


Q9D1C8

RefSeq (mRNA)


NM_016208 NM_183057


NM_025842 NM_001305668

RefSeq (protein)


NP_057292 NP_898880 NP_057292.1


NP_001292597 NP_080118

Location (UCSC)

Chr 8: 144.42 – 144.43 Mb

Chr 15: 76.51 – 76.51 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Vacuolar protein sorting-associated protein 28 homolog is a protein that in humans is encoded by the VPS28 gene.^[5]

Function

This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated protein. The two other subunits of ESCRT-I are vacuolar protein sorting 23 (VPS23), also known as tumor susceptibility gene 101 (TSG101), and vacuolar protein sorting 37 (VPS37). Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.^[5]

Interactions

VPS28 has been shown to interact with TSG101.^[6]^[7]^[8]

References

^ ^a ^b ^c ENSG00000285339 GRCh38: Ensembl release 89: ENSG00000160948, ENSG00000285339 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000115987 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b "Entrez Gene: VPS28 vacuolar protein sorting 28 homolog (S. cerevisiae)".
^ Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
^ Stuchell MD, Garrus JE, Müller B, Stray KM, Ghaffarian S, McKinnon R, Kräusslich HG, Morham SG, Sundquist WI (August 2004). "The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding". J. Biol. Chem. 279 (34): 36059–71. doi:10.1074/jbc.M405226200. PMID 15218037.
^ Bishop N, Woodman P (April 2001). "TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes". J. Biol. Chem. 276 (15): 11735–42. doi:10.1074/jbc.M009863200. PMID 11134028.

Raiborg C, Rusten TE, Stenmark H (2004). "Protein sorting into multivesicular endosomes". Curr. Opin. Cell Biol. 15 (4): 446–55. doi:10.1016/S0955-0674(03)00080-2. PMID 12892785.
Bishop N, Woodman P (2001). "TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes". J. Biol. Chem. 276 (15): 11735–42. doi:10.1074/jbc.M009863200. PMID 11134028.
Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (2001). "Protein–Protein Interaction Panel Using Mouse Full-Length cDNAs". Genome Res. 11 (10): 1758–65. doi:10.1101/gr.180101. PMC 311163. PMID 11591653.
Bishop N, Horman A, Woodman P (2002). "Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein–ubiquitin conjugates". J. Cell Biol. 157 (1): 91–101. doi:10.1083/jcb.200112080. PMC 2173266. PMID 11916981.
Martin-Serrano J, Zang T, Bieniasz PD (2003). "Role of ESCRT-I in Retroviral Budding". J. Virol. 77 (8): 4794–804. doi:10.1128/JVI.77.8.4794-4804.2003. PMC 152150. PMID 12663786.
Tanzi GO, Piefer AJ, Bates P (2003). "Equine Infectious Anemia Virus Utilizes Host Vesicular Protein Sorting Machinery during Particle Release". J. Virol. 77 (15): 8440–7. doi:10.1128/JVI.77.15.8440-8447.2003. PMC 165230. PMID 12857913.
Martin-Serrano J, Yarovoy A, Perez-Caballero D, Bieniasz PD, Yaravoy A (2003). "Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins". Proc. Natl. Acad. Sci. U.S.A. 100 (21): 12414–9. Bibcode:2003PNAS..10012414M. doi:10.1073/pnas.2133846100. PMC 218772. PMID 14519844.
Stuchell MD, Garrus JE, Müller B, Stray KM, Ghaffarian S, McKinnon R, Kräusslich HG, Morham SG, Sundquist WI (2005). "The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding". J. Biol. Chem. 279 (34): 36059–71. doi:10.1074/jbc.M405226200. PMID 15218037.
Bache KG, Slagsvold T, Cabezas A, Rosendal KR, Raiborg C, Stenmark H (2005). "The Growth-Regulatory Protein HCRP1/hVps37A Is a Subunit of Mammalian ESCRT-I and Mediates Receptor Down-Regulation". Mol. Biol. Cell. 15 (9): 4337–46. doi:10.1091/mbc.E04-03-0250. PMC 515363. PMID 15240819.
Eastman SW, Martin-Serrano J, Chung W, Zang T, Bieniasz PD (2005). "Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding". J. Biol. Chem. 280 (1): 628–36. doi:10.1074/jbc.M410384200. PMID 15509564.
Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.