Kompleks prepoznavanja mesta početka 4

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Kompleks prepoznavanja mesta početka, podjedinica 4
Identifikatori
SimboliORC4; ORC4L; ORC4P
Vanjski IDOMIM: 603056 MGI: 1347043 HomoloGene: 8059 GeneCards: ORC4 Gene
Ontologija gena
Molekulska funkcija • nucleotidno vezivanje
• vezivanje za meto početka DNK replikacije
• proteinsko vezivanje
Ćelijska komponenta • Kompleks prepoznavanja mesta početka
• nukleus
• nukleoplazma
Biološki proces • kontrolna tačka ćelijskog ciklusa
• G1/S tranzicija mitotičkog ćelijskog ciklusa
• S faza mitotičkog ćelijskog ciklusa
Pregled RNK izražavanja
podaci
Ortolozi
VrstaČovekMiš
Entrez500026428
EnsemblENSG00000115947ENSMUSG00000026761
UniProtO43929O88708
Ref. Sekv. (iRNK)NM_001190879NM_001177313
Ref. Sekv. (protein)NP_001177808NP_001170784
Lokacija (UCSC)Chr 2:
148.69 - 148.78 Mb		Chr 2:
48.9 - 48.95 Mb
PubMed pretraga[1][2]

Kompleks prepoznavanja mesta početka 4 je protein koji je kod ljudi kodiran ORC4 (ORC4L) genom.[1][2][3]

Kompleks prepoznavanja mesta početka (ORC) je visoko konzervirani proteinski kompleks sa šest podjedinica koji je esencijalan za inicijaciju replikacije DNK kod eukariotskih ćelija. Istraživanja na kvascu su pokazala da se ORC specifično vezuje za mesto početka replikacije i da služi kao platforma za konstrukciju prereplikacionog kompleksa, koji sadrži dodatne inicijacione faktore kao što su Cdc6 i Mcm proteini. Protein kodiran ovim genom je podjedinica kompleksa prepoznavanja mesta početka. Pokazano je da formira sržni kompleks sa ORC2L, -3L, i -5L. Tri alternativno splajsovane transkriptne varijante kodiraju isti protein.[3]

Interakcije

ORC4 mođe da formira interakcije sa ORC1,[4] MCM6,[4] ORC2,[1][4][5][6] ORC5,[4][5][7] ORC3,[5][6][8] MCM2,[4] MCM4,[4] MCM3[4] i ORC6.[4]

Reference

  1. ↑ 1,0 1,1 Quintana DG, Hou Zh, Thome KC, Hendricks M, Saha P, Dutta A (December 1997). „Identification of HsORC4, a member of the human origin of replication recognition complex”. J Biol Chem 272 (45): 28247–51. DOI:10.1074/jbc.272.45.28247. PMID 9353276.  Greška u referenci: Nevaljana oznaka <ref>; naziv "pmid9353276" je zadan više puta s različitim sadržajem
  2. ↑ Eki T, Dean FB, Kohda A, Okumura K, Abe M, Murakami Y, Ishiai M, Satomoto K, Hurwitz J, O'Donnell M, Hanaoka F (October 1998). „Assignment of the homologue of the yeast origin recognition complex subunit ORC4 (ORC4L) to human chromosome band 2q22→q23 by in situ hybridization and somatic cell hybrid analysis”. Cytogenet Cell Genet 81 (1): 89–90. DOI:10.1159/000014997. PMID 9691185. 
  3. ↑ 3,0 3,1 „Entrez Gene: ORC4L origin recognition complex, subunit 4-like (yeast)”. 
  4. ↑ 4,0 4,1 4,2 4,3 4,4 4,5 4,6 4,7 Kneissl, Margot; Pütter Vera, Szalay Aladar A, Grummt Friedrich (March 2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. (England) 327 (1): 111–28. DOI:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612. 
  5. ↑ 5,0 5,1 5,2 Dhar, S K; Delmolino L, Dutta A (August 2001). „Architecture of the human origin recognition complex”. J. Biol. Chem. (United States) 276 (31): 29067–71. DOI:10.1074/jbc.M103078200. ISSN 0021-9258. PMID 11395502. 
  6. ↑ 6,0 6,1 Vashee, S; Simancek P, Challberg M D, Kelly T J (July 2001). „Assembly of the human origin recognition complex”. J. Biol. Chem. (United States) 276 (28): 26666–73. DOI:10.1074/jbc.M102493200. ISSN 0021-9258. PMID 11323433. 
  7. ↑ Quintana, D G; Thome K C, Hou Z H, Ligon A H, Morton C C, Dutta A (October 1998). „ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases”. J. Biol. Chem. (UNITED STATES) 273 (42): 27137–45. DOI:10.1074/jbc.273.42.27137. ISSN 0021-9258. PMID 9765232. 
  8. ↑ Pinto, S; Quintana D G, Smith P, Mihalek R M, Hou Z H, Boynton S, Jones C J, Hendricks M, Velinzon K, Wohlschlegel J A, Austin R J, Lane W S, Tully T, Dutta A (May 1999). „latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant”. Neuron (UNITED STATES) 23 (1): 45–54. DOI:10.1016/S0896-6273(00)80752-7. ISSN 0896-6273. PMID 10402192. 

Literatura

  • Maruyama K, Sugano S (1994). „Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.”. Gene 138 (1–2): 171–4. DOI:10.1016/0378-1119(94)90802-8. PMID 8125298. 
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K i dr.. (1997). „Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library”. Gene 200 (1–2): 149–56. DOI:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
  • Tugal T, Zou-Yang XH, Gavin K i dr.. (1999). „The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p”. J. Biol. Chem. 273 (49): 32421–9. DOI:10.1074/jbc.273.49.32421. PMID 9829972. 
  • Springer J, Kneissl M, Pütter V, Grummt F (1999). „Identification and characterization of MmORC4 and MmORC5, two subunits of the mouse origin of replication recognition complex”. Chromosoma 108 (4): 243–9. DOI:10.1007/s004120050374. PMID 10460412. 
  • Jiang W, McDonald D, Hope TJ, Hunter T (1999). „Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication”. EMBO J. 18 (20): 5703–13. DOI:10.1093/emboj/18.20.5703. PMC 1171637. PMID 10523313. 
  • Thome KC, Dhar SK, Quintana DG i dr.. (2001). „Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins”. J. Biol. Chem. 275 (45): 35233–41. DOI:10.1074/jbc.M005765200. PMID 10954718. 
  • Vashee S, Simancek P, Challberg MD, Kelly TJ (2001). „Assembly of the human origin recognition complex”. J. Biol. Chem. 276 (28): 26666–73. DOI:10.1074/jbc.M102493200. PMID 11323433. 
  • Dhar SK, Delmolino L, Dutta A (2001). „Architecture of the human origin recognition complex”. J. Biol. Chem. 276 (31): 29067–71. DOI:10.1074/jbc.M103078200. PMID 11395502. 
  • Okuno Y, McNairn AJ, den Elzen N i dr.. (2001). „Stability, chromatin association and functional activity of mammalian pre-replication complex proteins during the cell cycle”. EMBO J. 20 (15): 4263–77. DOI:10.1093/emboj/20.15.4263. PMC 149150. PMID 11483529. 
  • Strausberg RL, Feingold EA, Grouse LH i dr.. (2003). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences”. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. DOI:10.1073/pnas.242603899. PMC 139241. PMID 12477932. 
  • Kneissl M, Pütter V, Szalay AA, Grummt F (2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. 327 (1): 111–28. DOI:10.1016/S0022-2836(03)00079-2. PMID 12614612. 
  • Ota T, Suzuki Y, Nishikawa T i dr.. (2004). „Complete sequencing and characterization of 21,243 full-length human cDNAs”. Nat. Genet. 36 (1): 40–5. DOI:10.1038/ng1285. PMID 14702039. 
  • Ramachandran N, Hainsworth E, Bhullar B i dr.. (2004). „Self-assembling protein microarrays”. Science 305 (5680): 86–90. DOI:10.1126/science.1097639. PMID 15232106. 
  • Gerhard DS, Wagner L, Feingold EA i dr.. (2004). „The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)”. Genome Res. 14 (10B): 2121–7. DOI:10.1101/gr.2596504. PMC 528928. PMID 15489334. 
  • Sibani S, Price GB, Zannis-Hadjopoulos M (2005). „Ku80 binds to human replication origins prior to the assembly of the ORC complex”. Biochemistry 44 (21): 7885–96. DOI:10.1021/bi047327n. PMID 15910003. 
  • Rual JF, Venkatesan K, Hao T i dr.. (2005). „Towards a proteome-scale map of the human protein-protein interaction network”. Nature 437 (7062): 1173–8. DOI:10.1038/nature04209. PMID 16189514. 
  • p
  • r
  • u
Separacija
i inicijacija
Prokariotska
(inicijacija)
Eukariotska
(priprema u
G1 fazi)
Oba
Replikacija
Prokariotska
(elongacija)
Eukariotska
(sinteza u
S fazi)
Oba
Kretanje: Procesivnost • DNK ligaza
Terminacija
B bsyn: dnk (repl, cycl, reco, repr) • tscr (fact, tcrg, nucl, rnat, rept, ptts) • tltn (risu, pttl, nexn) • dnab, rnab/runp • stru (domn, 1°, 2°, 3°, 4°)